标准编号：ISO 12966-1:2014
中文名称：动植物脂肪和油 脂肪酸甲酯的气相色谱 第1部分：脂肪酸甲基酯气相色谱法的指导原则
英文名称：Animal and vegetable fats and oils — Gas chromatography of fatty acid methyl esters — Part 1: Guidelines on modern gas chromatography of fatty acid methyl esters
发布日期：2014-12
标准范围
This part of ISO 12966 gives an overview of the gas chromatographic determination of fatty acids, freeand bound, in animal and vegetable fats and oils following their conversion to fatty acid methyl esters(FAMEs).The qualitative and quantitative determination of the composition of fatty acids by gas liquidchromatography (GLC) is a widely used application in lipid analysis. It is used for the characterization offats and oils, or fatty foodstuffs after the extraction of the oil from the matrix. The bound fatty acids ofthe triacylglycerols (TAGs) and, depending on the esterification method, the free fatty acids (FFA) andother lipids, are converted to fatty acid methyl esters (FAMEs), which are determined by capillary gaschromatography. Depending on the number of different fatty acids (theoretically more than 50 differentfatty acids can be present) capillary columns with a length of 10 m to 100 m are used for a separation.The GLC of FAMEs is applicable to all natural and synthetic mixtures of tri-, di- and monoacylglycerols,to fatty acid esters, free fatty acids, soaps and other fatty compounds. With this suite of standards,FAMEs from C4 to C26 can be determined, including saturated fatty acid methyl esters, cis- and transmonounsaturatedfatty acid methyl esters, and cis- and trans-polyunsaturated fatty acid methyl esters.For the determination of short chain fatty acids, isopropyl and butyl esters are often used so as to avoidinterferences with the solvent peak and in order to reduce differences in detector responses.
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