标准编号：ISO 16649-3:2015
中文名称：食物和动物饲料的微生物学-用于贝它-葡糖苷酸酶-阳性大肠杆菌计算的水平线方法 第3 部分： 采用5-溴-4 -氯-3-吲哚-贝它-D-葡糖苷酸酶的最大概率数值技术
英文名称：Microbiology of the food chain — Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli — Part 3: Detection and most probable number technique using 5-bromo-4-chloro-3-i
发布日期：2015-05
标准范围
This part of ISO 16649 specifies a horizontal method for the detection and enumeration of β-glucuronidasepositive Escherichia coli, by means of the liquid-medium culture technique and calculation of the mostprobable number (MPN) after incubation at (37 ± 1) °C, then at (44 ± 1) °C. This part of ISO 16649 isapplicable to the following:— products intended for human consumption and the feeding of animals;— environmental samples in the area of food production and food handling.The method is suitable for the enumeration of cells of E. coli that might have been subjected to stressarising from dehydration, freezing, and exposure to a saline (such as marine) environment or damageby disinfectants such as chlorine-containing products.A limitation of the applicability of this part of ISO 16649 is imposed by the susceptibility of the methodto a large degree of variability. The method is intended to be applied and the results interpreted in thelight of the information given in Clause 11.This method has not been fully evaluated for all matrices (e.g. for milk and milk products). ISO 7251 isintended to be used for milk and milk products.
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