标准编号：ISO/TS 21569-6:2016
中文名称：分子生物标志物分析的水平方法 检测转基因生物体和衍生产品的分析方法 第4部分：cry1Ab/Ac和Pubi-cry DNA序列检测用实时PCR筛选法
英文名称：Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 6: Real-time PCR based screening methods for t
发布日期：2016-11
标准范围
This document specifies a procedure for the detection of a DNA sequence of the modified cry1Ab/Acgene and a procedure for the detection of the DNA transition sequence between the maize ubiquitinpromoter (Pubi) and the cry1Ab/Ac gene. The modified cry1Ab/Ac gene and the Pubi-cry construct arefrequently found in genetically modified Bt plants. Both detection methods are based on real-time PCRand can be used for qualitative screening purposes. For identification and quantification of a specificgenetically modified plant (event) a follow-up analysis has to be carried out.This document is applicable for the analysis of DNA extracted from foodstuffs. It may also be suitable forthe analysis of DNA extracted from other products such as feedstuffs and seeds. The application of thesemethods requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix.
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