标准编号：ISO/TS 21569-5:2016
中文名称：分子生物标志物分析的水平方法 检测转基因生物体和衍生产品的分析方法 第4部分：FMV启动子（P-FMV）DNA序列检测用实时PCR筛选法
英文名称：Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 5: Real-time PCR based screening method for th
发布日期：2016-11
标准范围
This document specifies a procedure for the detection of a DNA sequence used in genetically modified(GM) plants by means of a real-time PCR (polymerase chain reaction). The method detects a 78 basepairs long segment of the Figwort mosaic virus 34S promoter DNA sequence. This segment in some GMplants is indicated as FMV promoter (P-FMV) and in other GM plants as FMV enhancer (E-FMV).The method was developed and validated for the analysis of DNA extracted from foodstuffs. It may besuitable also for analysis of other products such as feedstuffs and seeds. The procedure requires theextraction of an adequate quantity and quality of amplifiable DNA from the test sample.The DNA sequence amplified by the P-FMV element-specific method can be detected in samples whichcontain DNA of the naturally occurring Figwort mosaic virus. For this reason, it is necessary to confirma positive screening result. Further analyses are required using construct-specific or event specificmethods.
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