标准编号：ISO/TS 12869:2019
中文名称：水质 利用定量聚合酶链反应（QPCR）实现浓缩和基因扩增的方式检测和量化军团菌属和/或嗜肺军团菌
英文名称：Water quality — Detection and quantification of Legionella spp. and/or Legionella pneumophila by concentration and genic amplification by quantitative polymerase chain reaction (qPCR)
发布日期：2019-04
标准范围
This document specifies a method for the detection and quantification of Legionella spp. and
L. pneumophila using a quantitative polymerase chain reaction (qPCR). It specifies general
methodological requirements, performance evaluation requirements, and quality control requirements.
Technical details specified in this document are given for information only. Any other technical
solutions complying with the performance requirements are suitable.
NOTE 1 For performance requirements, see Clause 9.
This document is intended to be applied in the bacteriological investigation of all types of water (hot
or cold water, cooling tower water, etc.), unless the nature and/or content of suspended matter and/or
accompanying flora interfere with the determination. This interference can result in an adverse effect
on both the detection limit and the quantification limit.
NOTE 2 For validation requirements, see 9.7.
The results are expressed as the number of genome units of Legionella spp. and/or L. pneumophila per
litre of sample.
The method described in this document is applicable to all types of water. However, some additives, such
as chemicals used for water treatment, can interfere with and/or affect the sensitivity of the method.
The qPCR methods do not give any information about the physiological state of the Legionella.
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