标准编号：ISO/TS 16649-3:2005
中文名称：食物和动物饲料的微生物学-用于贝它-葡糖苷酸酶-阳性大肠杆菌计算的水平线方法 第3 部分： 采用5-溴-4 -氯-3-吲哚-贝它-D-葡糖苷酸酶的最大概率数值技术
英文名称：Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli — Part 3: Most probable number technique using 5-bromo-4-chloro-3
发布日期：2005-09
标准范围
This Technical Specification specifies a horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli, by means of the liquid-medium culture technique and calculation of the most probable number (MPN) after incubation at 37 ℃, then at 44 ℃.This Technical Specification is applicable to— products intended for human consumption and the feeding of animals, and— environmental samples in the area of food production and food handling.The method is suitable for the enumeration of cells of Escherichia coli that may have been subjected to stress arising from dehydration, freezing, exposure to a saline (such as marine) environment or damage by disinfectants such as chlorine-containing products.A limitation of the applicability of this Technical Specification is imposed by the susceptibility of the method to a large degree of variability. The method is to be applied and the results interpreted in the light of the information given in Clause 11.WARNING — Strains of Escherichia coli that do not grow at 44 ℃ and, in particular, those that are β-glucuronidase negative, such as Escherichia coli O157 and some other strains of pathogenic E. coli, will not be detected by the method described in this Technical Specification.
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