标准编号:ISO/TS 18867:2015

中文名称:食物链的微生物学 食源性病原体检测用聚合酶链反应(PCR) 致病性小肠结肠炎耶尔森氏菌和假结核耶尔森氏菌的检测

英文名称:Microbiology of the food chain — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Detection of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis

发布日期:2015-09

标准范围

This Technical Specification specifies two horizontal methods for detection of the pathogenicbioserotypes of Y. enterocolitica and one for detection of Y. pseudotuberculosis by using real-time PCRbasedmethods. The described methods allow for the detection of the two pathogens in enrichmentsand allow the isolation of colonies. Y. pestis, the causative agent of bubonic and pneumonic plagueharbours a variant of the ail gene as well and will be detected by the same primer/probe set as Y.pseudotuberculosis. However, Y. pestis is normally not associated with food. This Technical Specificationis applicable to products for human consumption, animal feeding stuffs, and environmental samples.

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