标准编号：ISO 23443:2020
中文名称：婴儿配方奶粉和成人营养品 反相超高高效液相色谱法（RP-UHPLC）测定胡萝卜素、番茄红素和叶黄素
英文名称：Infant formula and adult nutritionals — Determination of β-carotene, lycopene and lutein by reversed-phase ultra-high performance liquid chromatography (RP-UHPLC)
发布日期：2020-07
标准范围
This document specifies a method for the quantitative determination of β-carotene and lycopene in
infant formula and adult nutritionals in solid (i.e. powders) or liquid (i.e. ready-to-feed liquids and liquid concentrates) forms using reversed-phase ultra-high performance liquid chromatography (RP-UHPLC) and UV-visible detection. The application range runs from 1 μg/100 g to 1 500 μg/100 g for lycopene and from 1 μg/100 g to 2 250 μg/100 g for β-carotene. Based on the single-laboratory validation, the limit of detection (LOD) was 0,1 μg/100 g and the limit of quantification (LOQ) was 0,3 μg/100 g for each carotenoid.
The method does not apply to materials that contain measurable levels of β-apo-8′-carotenal. The
reproducibility data meets the requirements given in References [8] and [10].
Annex C specifies the determination of lutein. The reproducibility data does not meet the requirements given in Reference [9].
标准预览图
立即下载标准文件