标准编号:ISO 21446:2019

中文名称:婴儿配方奶粉和成人营养品 反式和总(cis + trans)维生素k1含量的测定 正常相高效液相色谱法

英文名称:Infant formula and adult nutritionals — Determination of trans and total (cis + trans) vitamin K1 content — Normal phase HPLC

发布日期:2019-01

标准范围

This document specifies a method for the quantitative determination of trans and total (cis + trans) vitamin K in infant, pediatric and adult nutritionals using normal phase (NP) high-performance 1 liquid chromatography (HPLC) with post-column reduction and fluorescence detection. The method demonstrated good linearity over a standard range of ~2 μg/l to 80 μg/l trans vitamin K , and the limit 1 of quantification (LOQ) was estimated to be 0,4 μg/l for standards and 0,09 μg/100 g ready to feed (RTF) for samples assuming 4 grams of sample are diluted to 10 ml.

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