ISO/TS 19006:2016 纳米技术 5 -（和6）- chloromethyl-2，7二氯醋酸二氢荧光素（cm-h2dcf-da） 纳米颗粒诱导的细胞内活性氧（ROS）在RAW 264.7巨噬细胞系生产检测的评估

标准编号：ISO/TS 19006:2016

中文名称：纳米技术 5 -（和6）- chloromethyl-2，7二氯醋酸二氢荧光素（cm-h2dcf-da） 纳米颗粒诱导的细胞内活性氧（ROS）在RAW 264.7巨噬细胞系生产检测的评估

英文名称：Nanotechnologies — 5-(and 6)-Chloromethyl-2’,7’ Dichloro-dihydrofluorescein diacetate (CM-H2DCF-DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in

发布日期：2016-08

标准范围

This document describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H2DCFDA assay.The protocol in this document is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.

标准预览图