标准编号：ISO 18744:2016
中文名称：食物链的微生物学 新鲜绿叶菜和浆果中隐孢子虫和贾第鞭毛虫的检测和计数
英文名称：Microbiology of the food chain — Detection and enumeration of Cryptosporidium and Giardia in fresh leafy green vegetables and berry fruits
发布日期：2016-04
标准范围
This International Standard specifies a method that is applicable for the detection and enumeration ofCryptosporidium oocysts and Giardia cysts on or in food products that are described herein as fresh leafygreen vegetables and berry fruits. With suitable controls, it may also be applicable for the examinationof other fresh produce.The microscopy descriptions are for Cryptosporidium spp. oocysts and Giardia duodenalis cysts of sizeranges which include those species (Cryptosporidium) or assemblages (Giardia) known to be pathogenicto humans.This method does not include any molecular analysis and therefore is not suitable for the determinationof the species or genotypes/assemblages of Cryptosporidium oocysts and Giardia cysts. The method willdetect all species and genotypes/assemblages that are known to be pathogenic for humans and alsoothers that are not. For further identification, molecular typing assays are required. However, thesecannot be reliably performed if process positive controls have been spiked into the samples, as theresult of molecular typing assays will be obfuscated.This method does not allow the determination of viability or infectivity of any Cryptosporidium oocystsand Giardia cysts which may be present.
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