标准编号：ISO/TS 20224-1:2020
中文名称：分子生物标记物分析 用实时PCR法检测食品和饲料中的动物源性材料 第1部分：牛DNA检测方法
英文名称：Molecular biomarker analysis — Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Part 1: Bovine DNA detection method
发布日期：2020-07
标准范围
This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of bovine-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of bovine material derived from cattle including taurine (Bos taurus) and zebu (Bos indicus). The assay also detects the species bison (Bison bison) and yak (Bos mutus).
The target sequence is a partial fragment of the bovine nuclear beta actin gene in chromosome 25 (i.e. [1][2][3] GenBank accession number NC_037352.1) , which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % replicability at this concentration (LOD ). 95 %
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