标准编号：ISO/TS 21569-2:2021
中文名称：分子生物标志物分析的水平方法 检测转基因生物体和衍生产品的分析方法 第2部分：构建特定的用于检测亚麻籽和亚麻籽产品中安全性未经审查转基因（FP967）事件的实时性PCR方法
英文名称：Molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 2: Construct-specific real-time PCR method for detection of event FP9
发布日期：2021-07
标准范围
This document specifies a procedure for the detection of a DNA sequence present in a genetically modified linseed (Linum usitatissimum) line (event FP967, also named as “CDC Triffid”). For this purpose, extracted DNA is used in a real-time PCR and the genetic modification (GM) is specifically detected by amplification of a 105 bp DNA sequence representing the transition between the nopaline synthase gene terminator (Tnos) from Agrobacterium tumefaciens and the dihydrofolate reductase gene (dfrA1) from a Class 1 integron of Escherichia coli.
The method described is applicable for the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix for the purpose of analysis.
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